| Product Name | Lck (phospho Tyr505) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Lck (phospho Tyr505)Lck is a membrane associated tyrosine protein kinases. The Lck gene has been shown to undergo rearrangement and overexpression in some murine lymphomas. In human studies, it has been demonstrated that the Lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas. Resting T cells contain high levels of the Lck protein and mRNA both of which decline upon activation of the T cells. These and other observations suggest that alterations in Lck expression may contribute to the pathogenesis of some types of neoplastic disease. |
| Modification | p-Tyr505 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIP |
| Western blotting (1-2 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (20-25 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 56 kDa phosphorylated Lck (Tyr505) of human, mouse and rat origins. Reactivity to other species has not been tested.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LCK phospho Lck (pY505) phospho Lck (pTyr505) phospho-Lck (pTyr505) p56-LCK phosphoLck (pY505) p56lck YT16 pp58lck phosphoLck (pTyr505) phospho-Lck (pY505) LSK p56 LCK Lck (phospho Y505) Lck (phospho Tyr505) Lck (pY505) Lck (pTyr505) Lck p |
|---|---|
| Target | Lck (phospho Tyr505) |
| Tissue Specificity | This antibody is specific for Lck (phospho Tyr505). |
| Cellular Localization | Present in lipid rafts in an unactive form. |
| Application Notes | Western blotting (1-2 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (20-25 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 56 kDa phosphorylated Lck (Tyr505) of human, mouse and rat origins. Reactivity to other species has not been tested.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Store at +4C. Product should remain stable for at least 12 months. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% sodium azide |
