Application
Leptin antibody detects Leptin protein by Western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract B. 30 µg MDA-MB-231 whole cell lysate/extract 12 % SDS-PAGE Leptin antibody dilution: 1:1000Application
Leptin antibody detects Leptin protein at secreted on human lung papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung papillary adenocarcinoma. Leptin antibody diluted at 1:250.| Product Name | Leptin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Leptin (leptin)This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq] |
| Immunogen | Full length human Leptin Recombinant protein. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Mouse, Rat, Dog, Cat, Pig, Rabbit, Rhesus Monkey, Chimpanzee, BovineMouse (83%), Rat (82%), Dog (80%), Cat (84%), Pig (85%), Rabbit (80%), Rhesus Monkey (89%), Chimpanzee (99%), Bovine (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | leptin LEPD OBS LEP OB |
|---|---|
| Target | Leptin |
| Cellular Localization | Secreted |
Application
Leptin antibody detects Leptin protein by Western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract B. 30 µg MDA-MB-231 whole cell lysate/extract 12 % SDS-PAGE Leptin antibody dilution: 1:1000
Application
Leptin antibody detects Leptin protein at secreted on human lung papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung papillary adenocarcinoma. Leptin antibody diluted at 1:250.| Positive Control | MCF-7 , MDA-MB-231 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
