Application
LETM1 antibody detects LETM1 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. LETM1 antibody diluted at 1:500.Application
LETM1 antibody detects LETM1 protein at cytoplasm on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. LETM1 antibody diluted at 1:500.Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed A431, using LETM1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using LETM1 antibody at 1:500 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | LETM1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LETM1 (leucine zipper-EF-hand containing transmembrane protein 1)This gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause Wolf-Hirschhorn syndrome, a complex malformation syndrome caused by the deletion of parts of the distal short arm of chromosome 4. Related pseudogenes have been identified on chromosomes 8, 15 and 19. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human LETM1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (83%), Bovine (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LETM1 antibody "LETM1 and EF-hand domain-containing protein 1 mitochondrial antibody" leucine zipper-EF-hand-containing transmembrane protein 1 antibody Mdm38 homolog antibody leucine zipper-EF-hand containing transmembrane protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein |
Application
LETM1 antibody detects LETM1 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. LETM1 antibody diluted at 1:500.
Application
LETM1 antibody detects LETM1 protein at cytoplasm on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. LETM1 antibody diluted at 1:500.
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed A431, using LETM1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using LETM1 antibody at 1:500 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
