Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:5000| Product Name | LGI1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LGI1 (leucine-rich, glioma inactivated 1)This gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobic segment representing a putative transmembrane domain with the amino terminus located outside the cell. It also contains leucine-rich repeats with conserved cysteine-rich flanking sequences. This gene is predominantly expressed in neural tissues and its expression is reduced in low grade brain tumors and significantly reduced or absent in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LGI1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (95%), Rat (95%), Chicken (86%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EPITEMPIN antibody EPT antibody ETL1 antibody IB1099 antibody LGI1 antibody epitempin-1 antibody leucine-rich glioma-inactivated protein 1 antibody "leucine-rich glioma inactivated 1 antibody" |
|---|---|
| Target | LGI1 |
| Molecular Weight(MW) | 64 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:5000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
