Application
LGR5 antibody detects LGR5 protein by western blot analysis. Non-transfected (-, unboiled) and LGR5-transfected (+, unboiled) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody diluted at 1:1000.| Product Name | LGR5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LGR5 (leucine-rich repeat containing G protein-coupled receptor 5)Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human LGR5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (84%), Rat (83%), Pig (90%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GRP49 leucine-rich repeat containing G protein-coupled receptor 5 FEX GPR67 GPR49 HG38 |
|---|---|
| Target | LGR5 |
| Molecular Weight(MW) | 100 kDa (note) (3) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein |
Application
LGR5 antibody detects LGR5 protein by western blot analysis. Non-transfected (-, unboiled) and LGR5-transfected (+, unboiled) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody diluted at 1:1000.| Positive Control | Myc-human LGR5-transfected 293T cells(un-boiled) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
