Application
Immunofluorescence staining of methanol-fixed Hela cells using LIMK2 (Phospho-Thr505) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK2 (Phospho-Thr505) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela cells untreated or treated with PMA using LIMK2 (Phospho-Thr505) Antibody .Application
Western blot analysis of extracts from Hela cells untreated or treated with PMA using LIMK2(Phospho-Thr505)| Product Name | LIMK2 (phospho Thr505) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LIMK2 (Phospho Thr505)Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from Human LIMK2. |
| Modification | p-Thr505 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LIMK2 LIM domain kinase 2 |
|---|---|
| Target | LIMK2 (Phospho Thr505) |
| Molecular Weight(MW) | 70 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using LIMK2 (Phospho-Thr505) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK2 (Phospho-Thr505) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela cells untreated or treated with PMA using LIMK2 (Phospho-Thr505) Antibody .
Application
Western blot analysis of extracts from Hela cells untreated or treated with PMA using LIMK2(Phospho-Thr505)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
