Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Lin28A antibody antibody at 1:500 dilution.Application
Sample (20 µg of whole cell lysate) A: mouse ESC 12% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Flow cytometry on human embryonic stem cells, staining with Lin28A antibody at 1:100 dilution(purple) or rabbit IgG (black).Application
Sample (20 µg of whole cell lysate) A: hESC 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Lin28A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Lin28A (lin-28 homolog A (C. elegans))Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LIN28. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMousePig |
| Predicted Reactivity | Rat, Cat, Chicken, Sheep, BovineRat (98%), Cat (96%), Chicken (82%), Sheep (98%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CSDD1 antibody FLJ12457 antibody LIN-28 antibody LIN28 antibody ZCCHC1 antibody LIN28A antibody zinc finger CCHC domain-containing protein 1 antibody protein lin-28 homolog A antibody "zinc finger CCHC domain containing 1 antibody" RNA-binding pr |
|---|---|
| Molecular Weight(MW) | 23 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus , nucleolus |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Lin28A antibody antibody at 1:500 dilution.
Application
Sample (20 µg of whole cell lysate) A: mouse ESC 12% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Flow cytometry on human embryonic stem cells, staining with Lin28A antibody at 1:100 dilution(purple) or rabbit IgG (black).
Application
Sample (20 µg of whole cell lysate) A: hESC 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | human ESC , mouse ESC , *pig heart tissue |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
