Application
Sample (30 µg of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
LMAN2 antibody [N1C2] detects LMAN2 protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: LMAN2 protein stained by LMAN2 antibody [N1C2] diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using LMAN2 antibody at 1:100 dilution.| Product Name | LMAN2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LMAN2 (lectin, mannose-binding 2)This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking and quality control. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LMAN2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, BovineMouse (99%), Rat (97%), Dog (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C5orf8 antibody, GP36B antibody, VIP36 antibody, LMAN2 antibody, glycoprotein GP36b antibody, vesicular integral-membrane protein VIP36 antibody, vesicular integral-membrane protein 36 antibody, vesicular integral protein of 36 kDa antibody, "lectin, manno |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein , Golgi apparatus membrane; Single-pass membrane protein , Endoplasmic reticulum membrane; Single-pass type I membrane protein |
Application
Sample (30 µg of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
LMAN2 antibody [N1C2] detects LMAN2 protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: LMAN2 protein stained by LMAN2 antibody [N1C2] diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using LMAN2 antibody at 1:100 dilution.| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
