Application
LRP130 antibody [N3C2], Internal detects LRP130 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LRP130 protein stained by LRP130 antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using LRP130 antibody at 1:100 dilution.| Product Name | LRP130 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LRP130 (leucine-rich PPR-motif containing)This gene encodes a protein that is leucine-rich and is thought to play a role in regulating the interaction of the cytoskeleton with a variety of cellular processes. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. Transcripts ranging in size from 4.8 to 7.0 kb which result from alternative polyadenylation have been reported for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LRP130. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CLONE-23970 antibody, GP130 antibody, LRP130 antibody, LSFC antibody, LRPPRC antibody, "leucine-rich PPR motif-containing protein, mitochondrial antibody", 130 kDa leucine-rich protein antibody, mitochondrial leucine-rich PPR motif-containing protein antib |
|---|---|
| Molecular Weight(MW) | 158 kDa (note) |
| Cellular Localization | Mitochondrion , Nucleus , nucleoplasm , Nucleus inner membrane , Nucleus outer membrane |
Application
LRP130 antibody [N3C2], Internal detects LRP130 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LRP130 protein stained by LRP130 antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using LRP130 antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
