Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000Application
LSM11 antibody detects LSM11 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LSM11 protein stained by LSM11 antibody diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | LSM11 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LSM11 (LSM11, U7 small nuclear RNA associated)Component of the U7 snRNP complex that is involved in the histone 3'-end pre-mRNA processing (By similarity). Increases U7 snRNA levels but not histone 3'-end pre-mRNA processing activity, when overexpressed. Required for cell cycle progression from G1 to S phases. Binds specifically to the Sm-binding site of U7 snRNA. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LSM11. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (96%), Rat (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ38273 antibody, LSM11 antibody, U7 snRNA-associated Sm-like protein LSm11 antibody, "LSM11 homolog, U7 small nuclear RNA associated antibody", 2210404M20Rik antibody, "LSM11, U7 small nuclear RNA associated antibody" |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000
Application
LSM11 antibody detects LSM11 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LSM11 protein stained by LSM11 antibody diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
