Rabbit Polyclonal antibody to LSM4 (LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae))Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM]
Immunogen
Recombinant protein encompassing a sequence within the center region of human LSM4. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFWB
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Zebrafish, Dog, Rhesus Monkey, BovineMouse (96%), Rat (96%), Zebrafish (82%), Dog (100%), Rhesus Monkey (100%), Bovine (100%)
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
YER112W antibody, LSM4 antibody, U6 snRNA-associated Sm-like protein LSm4 antibody, glycine-rich protein antibody, "LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae) antibody"
Molecular Weight(MW)
15 kDa (note) (2)
Cellular Localization
Nucleus
Application
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using LSM4 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 15% SDS PAGE diluted at 1:1000
Positive Control
A549 , HeLa , HepG2 , HCT116
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.