Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using LYAG antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using LYAG antibody at 1:500 dilution.| Product Name | LYAG antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LYAG (glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II))This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human LYAG. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LYAG antibody, GAA antibody, lysosomal alpha-glucosidase antibody, aglucosidase alfa antibody, acid maltase antibody, "glucosidase, alpha, acid antibody" |
|---|---|
| Target | LYAG |
| Molecular Weight(MW) | 105 kDa (note) (3) |
| Cellular Localization | Lysosome |
Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using LYAG antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using LYAG antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
