Application
mAChR M3 antibody detects mAChR M3 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with mAChR M3 antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
mAChR M3 antibody detects mAChR M3 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: mAChR M3 protein stained by mAChR M3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | mAChR M3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to mAChR M3The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Muscarinic acetylcholine receptor M3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigMouse (86%), Rat (84%), Pig (95%), Rhesus Monkey (97%), Chimpanzee (100%), Bovine (92%), Guinea pig (84%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HM3 antibody, CHRM3 antibody, m3 muscarinic receptor antibody, muscarinic acetylcholine receptor M3 antibody, "cholinergic receptor, muscarinic 3 antibody" |
|---|---|
| Target | mAChR M3 |
| Molecular Weight(MW) | 66 kDa (note) |
| Cellular Localization | Cell membrane , Cell junction , synapse , postsynaptic cell membrane , Basolateral cell membrane |
Application
mAChR M3 antibody detects mAChR M3 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with mAChR M3 antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
mAChR M3 antibody detects mAChR M3 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: mAChR M3 protein stained by mAChR M3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
