Application
Macro H2A.2 antibody detects Macro H2A.2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human H2AFY2-transfected 293T cells 10 % SDS-PAGE Macro H2A.2 antibody dilution: 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Macro H2A.2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Macro H2A.2 antibody at 1:100 dilution.Application
Macro H2A.2 antibody [N3C3] detects Macro H2A.2 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Macro H2A.2 antibody [N3C3] diluted at 1:500.| Product Name | Macro H2A.2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Macro H2A.2 (H2A histone family, member Y2)Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Macro H2A.2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, Rhesus Monkey, BovineRat (99%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | macroH2A2 antibody, H2AFY2 antibody, core histone macro-H2A.2 antibody, mH2A2 antibody, core histone macroH2A2.2 antibody, histone macroH2A2 antibody, "H2A histone family, member Y2 antibody" |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Macro H2A.2 antibody detects Macro H2A.2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human H2AFY2-transfected 293T cells 10 % SDS-PAGE Macro H2A.2 antibody dilution: 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Macro H2A.2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Macro H2A.2 antibody at 1:100 dilution.
Application
Macro H2A.2 antibody [N3C3] detects Macro H2A.2 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Macro H2A.2 antibody [N3C3] diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
