Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [N1C3] diluted at 1:2000.Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000| Product Name | MAD2L1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MAD2L1 (MAD2 mitotic arrest deficient-like 1 (yeast))MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAD2L1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (94%), Rat (95%), Xenopus laevis (81%), Chicken (87%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HSMAD2 antibody, MAD2 antibody, MAD2L1 antibody, "MAD2 (mitotic arrest deficient, yeast, homolog)-like 1 antibody", MAD2-like protein 1 antibody, mitotic spindle assembly checkpoint protein MAD2A antibody, "mitotic arrest deficient, yeast, homolog-like 1 a |
|---|---|
| Target | MAD2 |
| Molecular Weight(MW) | 24 kDa (note) (2) |
| Cellular Localization | Nucleus , Kinetochore , Cytoplasm |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [N1C3] diluted at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
