Application
MAGE 1 antibody [N1C3] detects MAGE 1 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAGE 1 protein stained by MAGE 1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000| Product Name | MAGE 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MAGE 1 (melanoma antigen family A, 1 (directs expression of antigen MZ2-E))This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAGE 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CT1.1 antibody, MAGE1 antibody, MGC9326 antibody, MAGEA1 antibody, melanoma antigen family A 1 antibody, melanoma-associated antigen MZ2-E antibody, melanoma-associated antigen 1 antibody, MAGE-1 antigen antibody, "cancer/testis antigen family 1, member 1 |
|---|---|
| Target | MAGE 1 |
| Molecular Weight(MW) | 34 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
MAGE 1 antibody [N1C3] detects MAGE 1 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAGE 1 protein stained by MAGE 1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
