Application
Immunohistochemical analysis of paraffin-embedded SCC25 xenograft, using MAN1B1 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE MAN1B1 antibody diluted at 1:1000Application
MAN1B1 antibody [C1C3] detects MAN1B1 protein at cytoplasm on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. MAN1B1 antibody [C1C3] diluted at 1:500.Application
MAN1B1 antibody [C1C3] detects MAN1B1 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MAN1B1 antibody [C1C3] diluted at 1:500.| Product Name | MAN1B1 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MAN1B1 (mannosidase, alpha, class 1B, member 1)Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man(5-6)GlcNAc(2). |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAN1B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERMan1 antibody, MANA-ER antibody, MAN1B1 antibody, "ER alpha 1,2-mannosidase antibody", "endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase antibody", Man9GlcNAc2-specific processing alpha-mannosidase antibody, "endoplasmic reticulum man |
|---|---|
| Target | MAN1B1 |
| Molecular Weight(MW) | 80 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded SCC25 xenograft, using MAN1B1 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE MAN1B1 antibody diluted at 1:1000
Application
MAN1B1 antibody [C1C3] detects MAN1B1 protein at cytoplasm on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. MAN1B1 antibody [C1C3] diluted at 1:500.
Application
MAN1B1 antibody [C1C3] detects MAN1B1 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MAN1B1 antibody [C1C3] diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
