Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 10% SDS PAGE diluted at 1:1000Application
MANEA antibody detects MANEA protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. MANEA antibody diluted at 1:500.| Product Name | MANEA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MANEA (mannosidase, endo-alpha)N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MANEA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686D20120 antibody, ENDO antibody, FLJ12838 antibody, hEndo antibody, MANEA antibody, mandaselin antibody, "alpha 1,2-endomannosidase antibody", "glycoprotein endo-alpha-1,2-mannosidase antibody", endomannosidase antibody, endo-alpha mannosidase antib |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (2) |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 10% SDS PAGE diluted at 1:1000
Application
MANEA antibody detects MANEA protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. MANEA antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
