Application
MARS antibody [N3C2], Internal detects MARS protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MARS protein stained by MARS antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal diluted at 1:1000.Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal diluted at 1:1000.| Product Name | MARS antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MARS (methionyl-tRNA synthetase)Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MARS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Rhesus Monkey, BovineXenopus laevis (81%), Rhesus Monkey (97%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ35667 antibody METRS antibody MRS antibody MTRNS antibody MARS antibody cytosolic methionyl-tRNA synthetase antibody "methionyl-tRNA synthetase cytoplasmic antibody" "methionine tRNA ligase 1 cytoplasmic antibody" methionyl-tRNA synthetase ant |
|---|---|
| Target | MARS |
| Molecular Weight(MW) | 101 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
MARS antibody [N3C2], Internal detects MARS protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MARS protein stained by MARS antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal diluted at 1:1000.
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal diluted at 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
