Application
MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MCD antibody [N2C1], Internal dilution: 1:500.Application
MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. MCD antibody [N2C1], Internal dilution: 1:500.Application
MCD antibody detects MCD protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 7.5% SDS-PAGE MCD antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MCD antibody detects MCD protein by western blot analysis. A. 30 µg C8D30 whole cell lysate/extract 7.5% SDS-PAGE MCD antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCD antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using MCD antibody at 1:500 dilution.| Product Name | MCD antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCD (malonyl-CoA decarboxylase)The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MCD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (93%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MCD antibody MGC59795 antibody MLYCD antibody malonyl coenzyme A decarboxylase antibody "malonyl-CoA decarboxylase mitochondrial antibody" malonyl-CoA decarboxylase antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) |
| Cellular Localization | Mitochondrion , Cytoplasm , Peroxisome |
Application
MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MCD antibody [N2C1], Internal dilution: 1:500.
Application
MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. MCD antibody [N2C1], Internal dilution: 1:500.
Application
MCD antibody detects MCD protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 7.5% SDS-PAGE MCD antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MCD antibody detects MCD protein by western blot analysis. A. 30 µg C8D30 whole cell lysate/extract 7.5% SDS-PAGE MCD antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCD antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using MCD antibody at 1:500 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , C8D30 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
