Application
Western blot analysis of extracts of various cell lines, using MCM2 antibody.Application
Immunohistochemistry of paraffin-embedded human normal colon using MCM2 antibody at dilution of 1:200 (400x lens).| Product Name | MCM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCM2The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012] |
| Immunogen | Recombinant protein of human MCM2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BM28 MITOTIN D3S3194 minichromosome maintenance complex component 2 CCNL1 cdc19 CDCL1 |
|---|---|
| Target | MCM2 |
| Molecular Weight(MW) | 102 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using MCM2 antibody.
Application
Immunohistochemistry of paraffin-embedded human normal colon using MCM2 antibody at dilution of 1:200 (400x lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
