Application
ApplicationApplication
HeLa cell RIPA extract (50 mg) incubated with gtx24460 used at 0.2 mg/ml for 1 hour| Product Name | MCM3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MCM3The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq] |
| Immunogen | Synthetic peptide (Human) - 38 amino acids which represent a portion of human MCM 3 encoded in part by exons 10 and 11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: Use at a concentration of 2 - 5 μg/mg of lysate. WB: Use at a dilution of 1/2000 - 1/10000. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HCC5 MGC1157 P1-MCM3 P1.h RLFB P1 MCM3 P1MCM3 |
|---|---|
| Target | MCM3 |
Application
Application
Application
HeLa cell RIPA extract (50 mg) incubated with gtx24460 used at 0.2 mg/ml for 1 hour| Application Notes | IP: Use at a concentration of 2 - 5 μg/mg of lysate. WB: Use at a dilution of 1/2000 - 1/10000. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide |
