Application
Western blot analysis of extracts of various cell lines, using MCM3 antibody.Application
Immunohistochemistry of paraffin-embedded human oophoroma using MCM3 antibody at dilution of 1:100 (400x lens).Application
Immunofluorescence analysis of HeLa cell using MCM3 antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of U2OS cell using MCM3 antibody. Blue: DAPI for nuclear staining.| Product Name | MCM3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCM3The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] |
| Immunogen | Recombinant protein of human MCM3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | minichromosome maintenance complex component 3 P1MCM3 HCC5 P1.h RLFB |
|---|---|
| Target | MCM3 |
Application
Western blot analysis of extracts of various cell lines, using MCM3 antibody.
Application
Immunohistochemistry of paraffin-embedded human oophoroma using MCM3 antibody at dilution of 1:100 (400x lens).
Application
Immunofluorescence analysis of HeLa cell using MCM3 antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of U2OS cell using MCM3 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
