Application
Western blot analysis of MCM4 (pS54) expression in LO2 EGF-treated (A); HeLa (B); NIH3T3 (C); SP2/0 EGF-treated (D); H9C2 EGF-treated (E) whole cell lysates.| Product Name | MCM4 (phospho Ser54) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCM4 (phospho Ser54)The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MCM4. The exact sequence is proprietary. |
| Modification | p-Ser54 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | minichromosome maintenance complex component 4 CDC21 MCM4 NKGCD P1-CDC21 CDC54 hCdc21 NKCD |
|---|---|
| Target | MCM4 (phospho Ser54) |
| Tissue Specificity | Recognizes endogenous levels of MCM4 (pS54) protein. |
Application
Western blot analysis of MCM4 (pS54) expression in LO2 EGF-treated (A); HeLa (B); NIH3T3 (C); SP2/0 EGF-treated (D); H9C2 EGF-treated (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
