Application
Western blot analysis of extracts of various cell lines, using MCM4 antibody.Application
Immunohistochemistry of paraffin-embedded Human brain tissue using MCM4 antibody.Application
Immunohistochemistry of paraffin-embedded human B cell lymphoma using MCM4 antibody at dilution of 1:200 (400x lens).Application
Immunohistochemistry of paraffin-embedded rat pancreas using MCM4 antibody at dilution of 1:200 (400x lens).Application
Immunohistochemistry of paraffin-embedded mouse brain using MCM4 antibody at dilution of 1:200 (400x lens).Application
Immunohistochemistry of paraffin-embedded human stomach cancer using MCM4 antibody at dilution of 1:200 (400x lens).| Product Name | MCM4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCM4The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide of human MCM4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IP 1:20 - 1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDC54 minichromosome maintenance complex component 4 CDC21 NKGCD P1-CDC21 NKCD hCdc21 |
|---|---|
| Target | MCM4 |
| Molecular Weight(MW) | 97 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using MCM4 antibody.
Application
Immunohistochemistry of paraffin-embedded Human brain tissue using MCM4 antibody.
Application
Immunohistochemistry of paraffin-embedded human B cell lymphoma using MCM4 antibody at dilution of 1:200 (400x lens).
Application
Immunohistochemistry of paraffin-embedded rat pancreas using MCM4 antibody at dilution of 1:200 (400x lens).
Application
Immunohistochemistry of paraffin-embedded mouse brain using MCM4 antibody at dilution of 1:200 (400x lens).
Application
Immunohistochemistry of paraffin-embedded human stomach cancer using MCM4 antibody at dilution of 1:200 (400x lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IP 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
