Application
MCM6 antibody detects MCM6 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE MCM6 antibody dilution: 1:1000Application
MCM6 antibody detects MCM6 protein at nucleus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM6 protein stained by MCM6 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
MCM6 antibody detects MCM6 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. MCM6 antibody dilution: 1:500.Application
MCM6 antibody detects MCM6 protein at nucleus on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. MCM6 antibody dilution: 1:500.| Product Name | MCM6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCM6 (minichromosome maintenance complex component 6)The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human MCM6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, BovineMouse (97%), Rat (96%), Chicken (89%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MCG40308 Mis5 minichromosome maintenance complex component 6 P105MCM |
|---|---|
| Target | MCM6 |
| Molecular Weight(MW) | 93 kDa (note) |
| Cellular Localization | Nucleus |
Application
MCM6 antibody detects MCM6 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE MCM6 antibody dilution: 1:1000
Application
MCM6 antibody detects MCM6 protein at nucleus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM6 protein stained by MCM6 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
MCM6 antibody detects MCM6 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. MCM6 antibody dilution: 1:500.
Application
MCM6 antibody detects MCM6 protein at nucleus on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. MCM6 antibody dilution: 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
