Application
MCT1 antibody detects MCT1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCT1 antibody diluted by 1:1000.Application
Immunoprecipitation of MCT1 protein from HepG2 whole cell extracts using 5 μg of MCT1 antibody . Western blot analysis was performed using MCT1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
MCT1 antibody detects MCT1 protein by Western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg whole cell extract of human MCT1-transfected 293T cells 7.5 % SDS-PAGE MCT1 antibody dilution: 1:5000| Product Name | MCT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCT1 (solute carrier family 16, member 1 (monocarboxylic acid transporter 1))The SLC16A1 gene encodes a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT (Garcia et al., 1994 [PubMed 8124722]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MCT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (96%), Chimpanzee (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | solute carrier family 16 member 1 (monocarboxylic acid transporter 1) HHF7 SLC16A1 MCT |
|---|---|
| Target | MCT1 |
| Molecular Weight(MW) | 54 kDa (note) (2) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein |
Application
MCT1 antibody detects MCT1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCT1 antibody diluted by 1:1000.
Application
Immunoprecipitation of MCT1 protein from HepG2 whole cell extracts using 5 μg of MCT1 antibody . Western blot analysis was performed using MCT1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
MCT1 antibody detects MCT1 protein by Western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg whole cell extract of human MCT1-transfected 293T cells 7.5 % SDS-PAGE MCT1 antibody dilution: 1:5000| Positive Control | 293T , A431 , HeLa , HepG2 , human MCT1-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Data sheet for OM262740