Application
MDFIC Antibody (N-term) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human spleen reacted with MDFIC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of MDFIC Antibody (N-term) in Hela cell line lysates (35ug/lane). MDFIC (arrow) was detected using the purified Pab.| Product Name | MDFIC antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MDFIC (N-term)This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p4 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p4 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 79~108 amino acids from the N-terminal region of human MDFIC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Imfa domaincontaining protein HIC I-mfa domain-containing protein MyoD family inhibitor domain-containing protein MDFIC MyoD family inhibitor domaincontaining protein |
|---|---|
| Target | MDFIC |
| Molecular Weight(MW) | 37.042 kDa (note) |
| Cellular Localization | Also shows a granular distribution in the cytoplasm. Weak expression in the nucleus. |
Application
MDFIC Antibody (N-term) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human spleen reacted with MDFIC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of MDFIC Antibody (N-term) in Hela cell line lysates (35ug/lane). MDFIC (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
