Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (Phospho-Ser166) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells untreated or treated with Hydroxyurea using MDM2 (phospho-Ser166) Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells using MDM2 (phospho-Ser166) Antibody .Application
Western blot analysis of extracts from 293 cells untreated or treated with Hydroxyurea using MDM2 (phospho-Ser166) Antibody| Product Name | MDM2 (phospho Ser166) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MDM2 (Phospho Ser166)This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues |
| Immunogen | Peptide sequence around phosphorylation site of Serine 166 (A-I-S(p)-E-T) derived from Human MDM2. |
| Modification | p-Ser166 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HDMX MGC5370 MGC71221 hdm2 MDM2 Mdm2 transformed 3T3 cell double minute 2 p53 binding protein MDM2 variant FB30 double minute 2 human homolog of p53-binding protein MDM2 variant FB28 E3 ubiquitin-protein ligase Mdm2 oncoprotein Mdm2 Mdm2 p5 |
|---|---|
| Target | MDM2 (Phospho Ser166) |
| Molecular Weight(MW) | 90 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (Phospho-Ser166) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells untreated or treated with Hydroxyurea using MDM2 (phospho-Ser166) Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells using MDM2 (phospho-Ser166) Antibody .
Application
Western blot analysis of extracts from 293 cells untreated or treated with Hydroxyurea using MDM2 (phospho-Ser166) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
