Application
Western blot analysis of extracts from 293 and Hela cells using MDM2 Antibody .| Product Name | MDM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MDM2MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 . |
| Immunogen | Peptide sequence around aa.18~22(Q-I-P-A-S) derived from Human MDM2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HDMX MGC5370 MGC71221 hdm2 MDM2 Mdm2 transformed 3T3 cell double minute 2 p53 binding protein MDM2 variant FB30 double minute 2 human homolog of p53-binding protein MDM2 variant FB28 E3 ubiquitin-protein ligase Mdm2 oncoprotein Mdm2 Mdm2 p5 |
|---|---|
| Target | MDM2 |
| Molecular Weight(MW) | 90 (note) |
Application
Western blot analysis of extracts from 293 and Hela cells using MDM2 Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
