Application
MDMX antibody detects MDMX protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MDMX antibody diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using MDMX antibody at 1:500 dilution.Application
MDMX antibody detects MDM4 protein at nucleus on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. MDMX antibody dilution: 1:500.Application
MDMX antibody detects MDM4 protein at nucleus on SNU-1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SNU-1 xenograft. MDMX antibody dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | MDMX antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MDMX (protein Mdm4)The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MDMX. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chimpanzee, BovineRat (83%), Chimpanzee (99%), Bovine (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp781B1423 antibody HDMX antibody MDMX antibody MGC132766 antibody MRP1 antibody MDM4 antibody mdm2-like p53-binding protein antibody protein Mdm4 antibody "double minute 4 human homolog of p53-binding protein antibody" protein Mdmx antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) |
| Cellular Localization | Nucleus |
Application
MDMX antibody detects MDMX protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MDMX antibody diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using MDMX antibody at 1:500 dilution.
Application
MDMX antibody detects MDM4 protein at nucleus on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. MDMX antibody dilution: 1:500.
Application
MDMX antibody detects MDM4 protein at nucleus on SNU-1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SNU-1 xenograft. MDMX antibody dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
