Application
ME2 antibody detects ME2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME2 antibody diluted at a dilution of 1:1000.Application
Immunoprecipitation of ME2 protein from THP-1 whole cell extracts using 5 μg of ME2 antibody . Western blot analysis was performed using ME2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
ME2 antibody detects ME2 protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ME2 antibody diluted at 1:500.| Product Name | ME2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ME2 (malic enzyme 2, NAD(+)-dependent, mitochondrial)This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human ME2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (87%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ODS1 malic enzyme 2 NAD(+)-dependent mitochondrial |
|---|---|
| Target | ME2 |
| Molecular Weight(MW) | 65 kDa (note) |
| Cellular Localization | Mitochondrion matrix |
Application
ME2 antibody detects ME2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME2 antibody diluted at a dilution of 1:1000.
Application
Immunoprecipitation of ME2 protein from THP-1 whole cell extracts using 5 μg of ME2 antibody . Western blot analysis was performed using ME2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
ME2 antibody detects ME2 protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ME2 antibody diluted at 1:500.| Positive Control | THP-1 , HL-60 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
