Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MED18 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MED18 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000| Product Name | MED18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MED18 (mediator complex subunit 18)MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MED18. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (97%), Rat (98%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ20045 antibody p28b antibody MED18 antibody TRAP/mediator complex subunit p28b antibody mediator of RNA polymerase II transcription subunit 18 antibody "mediator of RNA polymerase II transcription subunit 18 homolog antibody" mediator complex sub |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MED18 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MED18 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
