Application
MED8 antibody detects MED8 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MED8 protein stained by MED8 antibody diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:1000| Product Name | MED8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MED8 (mediator complex subunit 8)This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MED8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ARC32 antibody MGC17544 antibody MGC19641 antibody MED8 antibody activator-recruited cofactor 32 kDa component antibody "mediator of RNA polymerase II transcription subunit 8 homolog antibody" mediator of RNA polymerase II transcription subunit 8 an |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) |
| Cellular Localization | Nucleus |
Application
MED8 antibody detects MED8 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MED8 protein stained by MED8 antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:1000| Positive Control | U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
