Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2A antibody [C2C3], C-term diluted at 1:500.Application
MEF2A antibody [C2C3], C-term detects MEF2A protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. MEF2A antibody [C2C3], C-term dilution: 1:500.Application
MEF2A antibody [C2C3], C-term detects MEF2A protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. MEF2A antibody [C2C3], C-term dilution: 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MEF2A antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000| Product Name | MEF2A antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEF2A (myocyte enhancer factor 2A)The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human MEF2A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (84%), Rhesus Monkey (95%), Bovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADCAD1 antibody RSRFC4 antibody RSRFC9 antibody MEF2A antibody serum response factor-like protein 1 antibody myocyte-specific enhancer factor 2A antibody "MADS box transcription enhancer factor 2 polypeptide A (myocyte enhancer factor 2A) antibody" |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2A antibody [C2C3], C-term diluted at 1:500.
Application
MEF2A antibody [C2C3], C-term detects MEF2A protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. MEF2A antibody [C2C3], C-term dilution: 1:500.
Application
MEF2A antibody [C2C3], C-term detects MEF2A protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. MEF2A antibody [C2C3], C-term dilution: 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MEF2A antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000| Positive Control | A431 , H1299 , HeLa , HepG2 , PC-12 , Rat-2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
