Application
MEF2C antibody detects MEF2C protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MEF2C protein stained by MEF2C antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
MEF2C antibody immunoprecipitates MEF2C protein in IP experiments. IP samples: K562 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of MEF2C protein by 4 μg MEF2C antibody 5 % SDS-PAGE The immunoprecipitated MEF2C protein was detected by MEF2C antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
MEF2C antibody detects MEF2C protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. MEF2C antibody dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MEF2C antibody detects MEF2C protein at nucleus on Saos2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Saos2 xenograft. MEF2C antibody dilution: 1:500.| Product Name | MEF2C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEF2C (myocyte enhancer factor 2C)This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MEF2C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevis, Pig, Sheep, Rhesus Monkey, BovineXenopus laevis (90%), Pig (97%), Sheep (97%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C5DELq14.3 DEL5q14.3 antibody MEF2C antibody myocyte-specific enhancer factor 2C antibody "MADS box transcription enhancer factor 2 polypeptide C antibody" myocyte enhancer factor 2C antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
MEF2C antibody detects MEF2C protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MEF2C protein stained by MEF2C antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
MEF2C antibody immunoprecipitates MEF2C protein in IP experiments. IP samples: K562 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of MEF2C protein by 4 μg MEF2C antibody 5 % SDS-PAGE The immunoprecipitated MEF2C protein was detected by MEF2C antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
MEF2C antibody detects MEF2C protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. MEF2C antibody dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MEF2C antibody detects MEF2C protein at nucleus on Saos2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Saos2 xenograft. MEF2C antibody dilution: 1:500.| Positive Control | U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
