Application
Western blot analysis of MKK1 (pT286) expression in HEK293T PMA-treated (A); NIH3T3 UV-treated (B); C2C12 (C); PC12 PMA-treated (D); C6 (E) whole cell lysates.| Product Name | MEK1 (phospho Thr286) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEK1 (phospho Thr286)The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MKK1. The exact sequence is proprietary. |
| Modification | p-Thr286 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | mitogen-activated protein kinase kinase 1 MAP2K1 PRKMK1 MAPKK1 MEK1 MKK1 |
|---|---|
| Target | MEK1 (phospho Thr286) |
| Tissue Specificity | Recognizes endogenous levels of MKK1 (pT286) protein. |
Application
Western blot analysis of MKK1 (pT286) expression in HEK293T PMA-treated (A); NIH3T3 UV-treated (B); C2C12 (C); PC12 PMA-treated (D); C6 (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
