Application
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~45k MEK1 protein phosphorylated at Thr 386 using MEK1 (phospho Thr386) antibody .| Product Name | MEK1 (phospho Thr386) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MEK1 (phospho Thr386)Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 conjugated to KLH |
| Modification | p-Thr386 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKMK1 MAP2K1 MKK1 MAPKK1 MEK1 MEK1 (phospho T386) MEK1 (phospho Thr386) MEK1 (pT386) MEK1 (pThr386) MEK1 phospho T386 MEK1 phospho Thr386 Phospho MEK1 (T386) Phospho MEK1 (Thr386) Phospho MEK1 (pT386) Phospho MEK1 (pThr386) |
|---|---|
| Target | MEK1 (phospho Thr386) |
| Molecular Weight(MW) | 45 (note) |
Application
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~45k MEK1 protein phosphorylated at Thr 386 using MEK1 (phospho Thr386) antibody .| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
