Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HepG2 and PC12 cells using MEK1(Ab-291) Antibody| Product Name | MEK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEK1Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. |
| Immunogen | Peptide sequence around aa. 289~293 (P-R-T-P-G) derived from Human MEK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MAPKK1 MEK1 MKK1 PRKMK1 MAP2K1 protein kinase mitogen-activated kinase 1 (MAP kinase kinase 1) ERK activator kinase 1 MEK 1 MAP kinase kinase 1 MAPK/ERK kinase 1 dual specificity mitogen-activated protein kinase kinase 1 MAPKK 1 mitogen-activ |
|---|---|
| Target | MEK1 |
| Molecular Weight(MW) | 45 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HepG2 and PC12 cells using MEK1(Ab-291) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
