Application
Western Blot: MEK1/2 Phosphospecific Antibody [ - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.Application
Application| Product Name | MEK1/2 (phospho Ser218/222) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MEK1/2 (phospho Ser218/222)Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 conjugated to KLH |
| Modification | p-Ser218/222 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKMK1 MKK1 MAPKK1 MEK1 MAP2K1/2 MEK1/2 (phospho S218/S222) MEK1/2 (phospho Ser218/Ser222) MEK1/2 (pS218/pS222) MEK1/2 (pSer218/pSer222) MEK1/2 phospho S218/S222 MEK1/2 phospho Ser218/Ser222 Phospho MEK1/2 (S218/S222) Phospho MEK1/2 (Ser218/Ser |
|---|---|
| Target | MEK1/2 (phospho Ser218,222) |
| Molecular Weight(MW) | 45 (note) |
Application
Western Blot: MEK1/2 Phosphospecific Antibody [ - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.
Application
Application| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
