Application
WB analysis of NIH 3T3 cell lysate using MEK1/2 (phospho Ser218/222) antibody in serum starved (control) or incubated in the presence of serum (serum) for 5 minutes.| Product Name | MEK1/2 (phospho Ser218/222) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MEK1The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated ser 218 and 222 within human MEK1 and 2. |
| Modification | p-Ser218/222 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blotting: Max Dilution 1/1000 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | zebrafish, Xenopus laevis, Rat, Monkey, Dog, Chicken, Bovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Phospho MEK1 (pSer218/222) Phospho MEK1 (pS218/222) MEK1 Ser218/222 phospho MEK1 S218/222 phospho phospho Ser218/222 MEK1 PRKMK1 MEK1 phospho MKK1 phospho S218/222 MEK1 MEK1 phospho S218/222 MAP2K1 MEK1 phospho Ser218/222 |
|---|---|
| Target | Mek 1/2 (phospho Ser218/222) |
| Tissue Specificity | This antibody is specific for MEK 1 and MEK 2 when phosphorylated at serine 218 and 222. |
Application
WB analysis of NIH 3T3 cell lysate using MEK1/2 (phospho Ser218/222) antibody in serum starved (control) or incubated in the presence of serum (serum) for 5 minutes.| Application Notes | Western Blotting: Max Dilution 1/1000 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | 10mM HEPES pH7.5 containing 0.09% Sodium Azide 0.01% Bovine Serum Albumin 50% Glycerol |
