Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2 (Phospho-Thr394) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HepG2 and Hela cells untreated or treated with UV using MEK-2 (Phospho-Thr394) Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells using MEK-2 (Phospho-Thr394) Antibody .| Product Name | MEK2 (phospho Thr394) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEK-2 (Phospho Thr394)Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2. |
| Modification | p-Thr394 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ26075 MAPKK2 MEK2 MKK2 PRKMK2 MAP2K2 MAP kinase kinase 2 ERK activator kinase 2 mitogen-activated protein kinase kinase 2 p45 MAPK/ERK kinase 2 dual specificity mitogen-activated protein kinase kinase 2 mitogen-activated protein kinase kinas |
|---|---|
| Target | MEK-2 (Phospho Thr394) |
| Molecular Weight(MW) | 44 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2 (Phospho-Thr394) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HepG2 and Hela cells untreated or treated with UV using MEK-2 (Phospho-Thr394) Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells using MEK-2 (Phospho-Thr394) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
