Application
Immunofluorescence analysis of methanol-fixed HeLa, using MEK2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MEK2 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000| Product Name | MEK2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEK2 (mitogen-activated protein kinase kinase 2)The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MEK2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Chicken, BovineMouse (91%), Rat (92%), Dog (93%), Pig (93%), Chicken (91%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ26075 antibody MAPKK2 antibody MEK2 antibody MKK2 antibody PRKMK2 antibody MAP2K2 antibody MAP kinase kinase 2 antibody ERK activator kinase 2 antibody "mitogen-activated protein kinase kinase 2 p45 antibody" MAPK/ERK kinase 2 antibody dual s |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using MEK2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MEK2 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
