Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:500Application
Immunoprecipitation of MEN1 protein from 293T whole cell extracts using 5 μg of Menin antibody [N1C1] . Western blot analysis was performed using Menin antibody [N1C1] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | Menin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Menin (multiple endocrine neoplasia I)This gene encodes menin, a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of this protein is not known. Two messages have been detected on northern blots but the larger message has not been characterized. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Menin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, BovineMouse (97%), Rat (97%), Dog (99%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MEAI antibody MENIN antibody SCG2 antibody MEN1 antibody "endocrine adenomatosis multiple antibody" multiple endocrine neoplasia I antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (7) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:500
Application
Immunoprecipitation of MEN1 protein from 293T whole cell extracts using 5 μg of Menin antibody [N1C1] . Western blot analysis was performed using Menin antibody [N1C1] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
