Application
Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system. Other detection methods will yield similar results.| Product Name | Merlin (phospho Ser518) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Merlin (phospho Ser518)The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin). The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlin's similarity to ERMs suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane. The NF2 protein is highly expressed in human fibroblasts and detected as a single band of about 75kD. This antibody is specific for the phosphorylated form of human NF2 (Merlin). |
| Immunogen | Human NF2 / Merlin phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - TDMKRL(pS)MEIEK (aa 508-530). |
| Modification | p-Ser518 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SCH BANF ACN NF2 |
|---|---|
| Target | NF2 |
Application
Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system. Other detection methods will yield similar results.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
