Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Merlin(Ab-518) Antibody (left) or the same antibody preincubated with blocking peptide (right)Application
Western blot analysis of extracts from Mouse brain tissue and C6 cells using Merlin(Ab-518) Antibody| Product Name | Merlin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MerlinProbable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. |
| Immunogen | Peptide sequence around aa.516~520 (R-L-S-M-E) derived from Human Merlin. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACN BANF SCH NF2 moesin-ezrin-radixin-like protein moesin-ezrin-radixin like schwannomin neurofibromin-2 merlin neurofibromin 2 (bilateral acoustic neuroma) moesin-ezrin-radizin-like protein schwannomerlin neurofibromin 2 (merlin) |
|---|---|
| Target | Merlin |
| Molecular Weight(MW) | 69 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Merlin(Ab-518) Antibody (left) or the same antibody preincubated with blocking peptide (right)
Application
Western blot analysis of extracts from Mouse brain tissue and C6 cells using Merlin(Ab-518) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
