Application
MGAT3 antibody [N3C3] detects MGAT3 protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. MGAT3 antibody [N3C3] diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MGAT3 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using GnT-III antibody at 1:200 dilution.| Product Name | MGAT3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MGAT3 (mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase)There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MGAT3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, ChickenRat (97%), Chicken (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ43371 antibody GNT-III antibody GNT3 antibody MGC141943 antibody MGC142278 antibody MGAT3 antibody N-acetylglucosaminyltransferase III antibody "beta-1 4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase antibody" GlcNAc-T III antibod |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (2) |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
Application
MGAT3 antibody [N3C3] detects MGAT3 protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. MGAT3 antibody [N3C3] diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MGAT3 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using GnT-III antibody at 1:200 dilution.| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
