Application
Immunofluorescence analysis of methanol-fixed HeLa, using MGAT4A antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using MGAT4A antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000| Product Name | MGAT4A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MGAT4A (mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A)This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human MGAT4A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (94%), Rat (95%), Pig (97%), Chicken (90%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GNT-IV antibody GNT-IVA antibody MGAT4A antibody N-acetylglucosaminyltransferase IVa antibody "UDP-GlcNAc:a-1 3-D-mannoside b-1 4-acetylglucosaminyltransferase IV antibody" "mannosyl (alpha-1 3-)-glycoprotein beta-1 4-N-acetylglucosaminyltransferase |
|---|---|
| Molecular Weight(MW) | 62 kDa (note) (3) |
| Cellular Localization | Alpha-1 , 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A: Golgi apparatus membrane; Single-pass type II membrane protein , 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form: Secreted |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using MGAT4A antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using MGAT4A antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
