Application
Human Brain stained with anti-GluR1 antibody| Product Name | mGluR1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to mGluR1L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternative splice variants of the GRM1 gene have been described but their full-length nature has not been determined. |
| Immunogen | Synthetic peptide (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P: Use at a dilution of 1/50 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. GTX15303 demonstrates cytoplasmic staining. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Chicken, Mouse, Rat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GPRC1A GRM1A MGLUR1 MGLUR1A mGlu1 |
|---|---|
| Target | mGluR1 |
| Molecular Weight(MW) | 110 kDa (note) |
| Cellular Localization | Integral membrane protein |
Application
Human Brain stained with anti-GluR1 antibody| Positive Control | Brain |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/50 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. GTX15303 demonstrates cytoplasmic staining. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
