Application
Immunofluorescence analysis of methanol-fixed A431, using Metabotropic Glutamate Receptor 6 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Metabotropic Glutamate Receptor 6 antibody at 1:100 dilution.Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:H1299 7.5% SDS PAGE diluted at 1:500| Product Name | mGluR6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Metabotropic Glutamate Receptor 6 (glutamate receptor, metabotropic 6)L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Metabotropic Glutamate Receptor 6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CSNB1B antibody DKFZp686H1993 antibody GPRC1F antibody MGLUR6 antibody mGlu6 antibody GRM6 antibody metabotropic glutamate receptor 6 antibody "glutamate receptor metabotropic 6 antibody" |
|---|---|
| Molecular Weight(MW) | 95 kDa (note) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein |
Application
Immunofluorescence analysis of methanol-fixed A431, using Metabotropic Glutamate Receptor 6 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Metabotropic Glutamate Receptor 6 antibody at 1:100 dilution.
Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:H1299 7.5% SDS PAGE diluted at 1:500| Positive Control | 293T , A431 , H1299 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
